Begin with careful homogenization of shrimp samples using specialized equipment. This crucial 4-minute process ensures uniform consistency for accurate downstream analysis, setting the foundation for reliable pathogen detection. This process can be adjusted to the needs of each lab. It can be done by blending the whole shrimp body or grinding the target shrimp organs. For water and soil samples, they can be taken directly.

Utilize the advanced Auto-Pure Mini system for efficient nucleic acid extraction. This automated 35-minute process isolates high-quality DNA and RNA from the prepared samples, ensuring optimal purity and yield for subsequent PCR analysis. This instrument has also been optimized to extract water and soil samples.

Precisely load extracted nucleic acids into PCR plates or strips for analysis. This 10-minute step involves careful handling and accurate pipetting to ensure uniform sample distribution, critical for the reliability of the subsequent digital PCR process.

Employ the cutting-edge Digital PCR System for highly precise pathogen quantification. This fully automated 2-hour process partitions samples into thousands of nanoliter-sized droplets, performs PCR amplification, and analyzes each droplet individually, providing unparalleled accuracy in detecting and quantifying multiple shrimp pathogens simultaneously.

Process the digital PCR results using software for comprehensive data analysis and interpretation. This final step generates detailed reports on pathogen presence and concentration, enabling precise disease diagnosis and risk assessment, also provide predictive insights for proactive farm management and disease prevention strategies.